Composite
Part:BBa_K2201201:Design
Designed by: Svenja Vinke Group: iGEM17_Bielefeld-CeBiTec (2017-10-20)
Pyrrolysyl tRNA/aminoacyl-synthetase for the incorporation of propargyllysine
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 585
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1992
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 573
Illegal AgeI site found at 1667 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 247
Design Notes
The part was inserted in pSB1C3 via gibson assembly.
Source
Lemke lab Heidelberg